Trypanosomatids by Unknown
Author:Unknown
Language: eng
Format: epub
ISBN: 9781071602942
Publisher: Springer US
This function sets the display of the image such that values of 1000 map to black (0 on your 8-bit screen) and 15,000 maps to white (255 on your 8-bit screen). The particular values suitable for any set of images require careful consideration.
3.2.3 Automated Saving of Raw and Composite Images
Finally, it is convenient to automate the saving of the image with the raw data (as a tiff) and composite images for display (as png) (see Note 12). This script asks the user for a directory, then saves the raw data as a tiff and png images of the overlay and the green fluorescent channel alone.//Bring up a window for the user to select a directory
path=getDirectory("");
//Save the raw data as a tiff
name=getTitle();
saveAs("TIFF", path+name+".tif");
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